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Abstracts

68. Identification of exotic forest pests by are usually below ground for example to (susceptible) '45H29' (1 generation resistan­
st
metabarcoding from a unique trap root crops and seedlings of field crops It is ce) and 'CS2000' (2 generation resistance)
nd
L­O LAROUCHE J A B(RUB( AND L BER­ commonly controlled by synthetic insecticides were inoculated with diferent quantities of P.
NIER Centre de foresterie des Laurentides, Res- applied as soil or seed treatments before plan­ brassicae inoculum and monitored for yield
sources naturelles Canada, Service canadien ting This study focused on the wireworm po­ pods per plant and 1000­grain weight The
des forêts, 1055 du PEPS, Québec, QC G1V pulation in the flood plains of Lac Saint­Pierre field experiment was conducted over 2 years
4C7, Canada; (L.B, L.-O.L) Institut de Biologie QC (LSP) as some conventional agricultural in biosecure clubroot nurseries inoculated
Intégrative et des Systèmes, Université Laval, practices (i e tillage pesticides and fertiliZers with pathotype 5X or a mix of pathotypes 5X
1030 Avenue de la Médecine, Québec, QC G1V use) in the region have negatively afected the and 3H at 5x10 5x10 and 5x10 resting spo­
4
8
6
0A6, Canada. lake biodiversity and water quality Ob ective res per plant In the greenhouse experiment
of this pro ect is to evaluate the wireworm plants were inoculated with pathotypes 5X 3H
With the growing volume of international economic threshold in diferent crops and or a mixture of both at 1x10 1x10 and 1x10 6
3
4
trade we obser ve an increase in the number propose recommendations regarding the resting spores per plant In both the field and
and diversity of exotic pests introduced in insecticide use in the floodplain Firstly the greenhouse clubroot incidence and disease
North America When these pests become wireworm population was evaluated across severity index (DSI) increased along with
established it is generally dificult to eradicate corn soybean and forage commercial fields inoculum density; the highest levels of disease
them For this reason the early detection of in the LSP region Secondly the negative were obser ved in the susceptible hybrid while
a new incoming pest is a priority To do so efects on wireworm populations of growing the lowest were found in '45H29 ' Yield pods
high­throughput sequencing techniques buckwheat (Fagopyrum esculentum Moench ) per plant and 1000­grain weight decreased
were developed to simultaneously process was also tested Six species of wireworms were as the DSI increased in all hybrids Yield was
and screen a large number of samples These found (Agriotes sp Aeolus sp Dalopius sp afected by DSI and canola hybrid but not by
methods are cost­ and time saving while ver y Hypnoidus abbreviatus Limonius sp Mela­ pathotype Regression analysis indicated that
efective for large numbers of samples We notus sp ) and their population varied across under greenhouse conditions an increment of
applied these methods to the detection of diferent regions (St Barthelemy Pierreville La 1% in the DSI resulted in a decrease of 0 49%
emerging pests collected in insect traps and Visitation de l'�le ­Dupas and Baie­du­Febvre) in yield; under field conditions this percen­
aerial spore traps We designed a single trap in LSP Based on the cultivated crops in those tage was reduced to 0 26% While the rate of
and protocol for the simultaneous identifica­ regions the wireworm population level was yield reduction was similar among hybrids
tion of pathogenic fungi and invasive insects considerably higher in forage crops and corn overall yield losses were lower in the clubroot
from insect trap preser vative fluids We did the fields than in soybean fields In the buckwheat resistant hosts since clubroot was less severe
proof of concept that insect cells present in plots the initial wireworm populations were
preser vative liquids from insect traps can be not suficiently high to clearly determine its 71 Fungal pathogen emergence: an
used to identify insects We screened numer­ potential repressive efects Since the wi­ Ustilago maydis x Sporisorium reilianum
ous present and potentially introduced pests reworm population were below the economic model
in North America using Illumina sequencing thresholds for all scouted fields the usage of E R M STORFIE AND B J SAVILLE (B.J.S.)
technolog y with specific primers for the fungal insecticide seed coating could be reduced in Environmental and Life Sciences Graduate
ITS region and the COI gene of insects Hun­ the floodplain Program, Trent University; and (E.R.M.S.; B.J.S.)
dreds of samples collected in British Colum­ Forensic Science Program, Trent University,
bia Ontario Quebec and New Brunswick were Session 14. Student oral competi­ 2140 East Bank Drive, Peterborough, ON K9L
sequenced We detected the presence of the tion: Plant and Soil Health 0G2, Canada.
fungus Heterobasidion occidentale Otrosina
& Garbelotto in Sault Ste Marie (ON) and the 70. Effect of clubroot (Plasmodiophora The emergence of fungal hybrid pathogens
presence of the bark beetle Anysandrus maic­ brassicae) on yield of canola (Brassica threatens sustainable crop production world­
he Stark in Hamilton's (ON) 2019 sampling napus) wide To investigate hybridiZation the related
A BOTERO­RAM�REZ S F HWANG AND S E smut fungi Ustilago maydis and Sporisorium
69. Characterization of wireworm popula­ reilianum, were selected because they infect
tions in the floodplains of Lac Saint­Pierre STRELKOV Department of Agricultural, Food a common host (Zea mays) can hybridiZe
and Nutritional Science, University of Alberta,
P RAMANATHAN P SEGUIN ( SMEDBOL T Edmonton, AB T6G 2P5, Canada. and there are tools available for their analysis
ALLART AND V GRAVEL Department of Plant Hybrid dikaryons demonstrated filamen­
Science, McGill University, Macdonald Campus, Clubroot caused by Plasmodiophora bras­ tous growth on plates similar to U. maydis
21111 Lakeshore Road, Sainte-Anne-de-Belle­ sicae, is a ma or disease of canola (Brassica dikaryons but showed reduced virulence and
vue, QC H9X 3V9, Canada. coloniZation of Z. mays compared to both
napus) The aim of this study was to evaluate
the efect of clubroot development on the parental dikaryons To understand the hybrid's
Wireworms (Coleoptera Elateridae) are yield of canola under field and greenhouse limited pathogenic development select
serious agricultural pests across the world and conditions Three canola hybrids '45H31' virulence gene transcript levels were assessed
their spread increases consistently Damages during pathogenic time courses using RT­PCR

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