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Abstracts

Resources and Environmental Sciences, 334 ty of Agricultural and Food Sciences, University ment is to mitigate pathogens through genetic
Leon Johnson Hall, Montana State University, of Manitoba, 222 Agri-culture Building, Winni­ resistance In this study we conducted geno­
Bozeman, MT 59971; (R.D.C) Agriculture and peg, MB R3T2N2, Canada; (M.R.I.) Department me wide identification and characteriZation
Agri-Food Canada, Swift Current Research and of Plant Pathology, Faculty of Agriculture, Ban­ of the VQ (Valine­Glutamine) gene family in B.
Development Centre, PO Box 1030, 1 Airport gladesh Agricultural University, Bangladesh. napus To understand the function of B. napus
Road, Swift Current, SK, Canada S9H 3X2; VQ genes in response to blackleg disease we
(C.J.P.) Crop Development Centre, Department NADPH oxidase (Nox) genes are crucial for the overexpressed BnVQ7 (BnaA01g36880D also
of Plant Sciences, University of Saskatchewan, production Reactive Oxygen Species (ROS) known as MKS1 gene) in a blackleg­suscepti­
51 Campus Drive, Saskatoon, SK, Canada, S7N and are important for many fungi's pathoge­ ble canola variety Westar Overexpression of
5A8; (K.T.N.) Agriculture and Agri-Food Canada, nicity Genome investigation of Verticillium BnMKS1 in canola did not improve resistance
Brandon Research and Development Centre, dahliae revealed the existence of three Nox­re­ to blackleg disease at seedling stage Howe­
PO BOX 1000A, 2701 Grand Valley Road, Bran­ lated genes namely NoxA NoxB and NoxC ver transgenic canola plants overexpressing
don, MB, R7A 5Y3 Canada. Here we investigated the role of these genes in BnMKS1 displayed enhanced resistance to L.
the pathogenicity of V. dahliae In vitro expres­ maculans infection at the adult plant stage In
The wheat stem sawfly Cephus cinctus sion of NoxA NoxB and NoxC showed signifi­ addition we identified and characterised Bn­
Norton (Hymenoptera Cephidae) is a ma or cantly higher expression of NoxB in the highly NCED3 (9­cis­epoxycarotenoid dioxygenease)
pest of wheat (Triticum aestivum L ) in the than the weakly aggressive isolate of V. dahliae and BnTX1 (Trithorax­like factor) from B. napus
northern Great Plains where it is a constant afer treatment with diferent potato extracts Transgenic B. napus Westar plant overexpres­
threat in Montana and is resurging in Alberta More transcripts of NoxA were detected in the sing BnNCED3 which shows enhanced adult
Saskatchewan and North Dakota Adoption weakly than the highly aggressive isolate in plant resistance against blackleg infection
of solid­stemmed cultivars is an impor­ response to leaf and stem extracts Significant­ BnTX1 was confirmed to bind to the promoter
tant management tool for wheat growers; ly higher expression of NoxC was obser ved in region of BnNCED3 gene using chromatin
however the inconsistent pith expression first the highly aggressive isolate treated with stem immunoprecipitation (ChIP)­qPCR Howe ver
noted with the release of 'Rescue' has been extracts Single gene disruption mutants were overexpression of BnTX1 in transgenic plants
repeatedly obser ved in modern cultivars in generated for each of these genes to better displays decreased adult plant resistance
Canada Given the extensive hectares planted understanding of the role of these genes in in response to blackleg pathogen infection
to solid­stemmed wheat cultivars during the pathogenicity of the highly aggressive These results indicated that minor genes play
an outbreak identification of cultivars that isolates Knocking out of both noxa and noxb important roles in canola against blackleg
display stable stem solidness and grain yield genes significantly decreased pathogenicity disease
across a wide range of environments where of the fungus indicating the essential roles of
stem sawfly infestations occur is desirable We these genes for V. dahliae's against its hosts 24. Characterizing the common wheat
assessed eight solid­stemmed and two ho­ The penetration ability of both noxa and noxb accession KU168­2 for the stem rust
llow­stemmed spring wheat genotypes grown knock­out strains was drastically reduced resistance
across diverse environments for their response compared to the wild type Gene complemen­ J S SHARMA T FETCH B MCCALLUM M CHI
in stem solidness and yield using multiple tation restored the pathogenicity as well as pe­ S S XU AND C W HIEBERT Agriculture and
statistical models Study sites included netration capability of the fungus in both noxa Agri-Food Canada, Morden Research and De­
southern Alberta and Saskatchewan Montana and noxb mutants Our data revealed that velopment Centre, 101 Route 100, Morden, MB
and North Dakota Most models agreed that NoxA and NoxB genes play important roles in R6M 1Y5, Canada; (M.C.) Department of Plant
the genotypes 'Choteau' 'BW925' and 'Mott' the pathogenicity of V. dahliae These findings Pathology, China Agricultural University, No.2
consistently displayed high and stable stem shed more light on the biolog y of V. dahliae Yuanmingyuan West Road, Beijing, 100193,
solidness concomitant with high grain yield and the mechanisms of its pathogenicity China; (S.S.X.) USDA-ARS, Cereal Crops Research
'Choteau' and 'BW925' also consistently met Unit, Edward T. Schafer Agricultural Research
or exceeded the desired threshold of a 3 75/5 23. Functional characterization of minor Center, Fargo, ND 58102, U.S.A.
pith rating for optimum resistance whereas genes of Brassica napus in response to
'Mott' developed optimal pith at a specific blackleg disease Wheat stem rust caused by a biotrophic
(early) phenological stage when resistance to Z ZOU F LIU S HUANG AND W G D FER­ fungal pathogen Puccinia graminis f sp tritici
WSS infestation is critical The identified stable NANDO. Department of Plant Science, Universi­ (Pgt) is a historically devastating disease of
and ideal genotypes would be useful to en­ ty of Manitoba, Winnipeg MB, R3T 2N2, Canada. wheat With the ongoing evolution of virulent
hance germplasm development production Pgt races especially the Ug99 group (TTKSK
and adoption of spring wheat cultivars in the Blackleg mainly caused by fungal pathogen and 13 variants) there is potential for more
WSS prone regions Leptosphaeria maculans is one of the most destruction in the future Deployment of
devastating diseases in canola (Brassica napus efective resistance (R) genes in cultivars
22. Role of NOX genes in Verticillium L ) resulting in significant yield loss worldwide is the preferred approach to combat this
dahliae’s pathogenicity An important element for bottom line profit threat In the current study a genetic study
M SAYARI X ZHU M R ISLAM F DAAYF Facul­ and sustainability of the crop and environ­ was conducted on a common wheat double

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