Page 29 - TriSociety Virtual Event

5. Delineating the SAR pathway in com­ 6. Interactions between light quality M YOOSEFZADEH­NAJAFABADI AND M
mon hexaploid wheat and plant hormones signaling to con­ ESKANDARI Department of Plant Agriculture,

S SUN R LINNING Y ZHANG C HERRFURTH trol flower bud induction in day­neutral University of Guelph, Guelph, ON N1G 2W1,
I FEUSSNER AND G BAKKEREN (S.S, Y.Z., G.B.) strawberry Canada.
Department of Botany, University of British V SIDHU AND V GRAVEL Department of Plant
Columbia, 6270 University Blvd, Vancouver, BC Science, McGill University, Macdonald Campus, Soybean [Glycine max (L ) Merr ] is known as a
V6T 1Z4, Canada; (S.S., R.L., G.B.) Agriculture 21 111 Lakeshore Road, Sainte-Anne-de-Bellev­ ma or strategic crop with low historical genetic
and Agri-Food Canada, Summerland Research ue, QC H9X 3V9, Canada. gain for seed yield To increase the genetic
& Development Centre, Summerland, BC V0H gain of soybean yield a better understanding
1Z0, Canada; (C.H., I.F.) Plant Biochemistry, Light quality is the key factor that regulates of the genetic architectures of its related traits
Georg-August University, Goettingen, D-37077, flowering time phenological growth and such as hyperspectral reflectance and yield
Germany. consequently strawberr y fruit production component traits may be useful While recent
Mechanism that directs flowering time under advances in high throughput phenotyping
In response to biotic stressors plants mobiliZe diferent light quality is poorly understood in and genotyping techniques have equipped
metabolic compounds in defense Local day­neutral strawberr y cultivars despite their breeders to generate large reliable datasets
threats can quickly lead to a robust whole­ rising popularity In the present study we de­ using appropriate tools for analyZing big data
plant immune response or systemic acquired termined how light quality controls flower bud is a bottleneck especially in public breeding
resistance (SAR) A L­lysine catabolic pathway induction during transplant production for programs In this study we used two conven­
was recently characteriZed in Arabidopsis 'Albion' strawberr y We determined the tran­ tional genome­wide association study (GWAS)
where the metabolites Pipecolic acid (Pip) scriptional levels of flowering related genes methods along with support vector regression
and N­hydroxylated pipecolic acid (NHP) FLOWERING LOCUS T (FvFT1) and TERMINAL (SVR) and random forest (RF) as two machi­
produced by the reductase SAR­Deficient 4 FLOWER1 (FvTFL1) as well as the quantifi­ ne learning­mediated GWAS methods to
(AtSARD4) and Flavin­monooxygenase 1 (AtF­ cation of the plant hormones Gibberellins evaluate the associations between yield and
MO1) respectively are crucial for proper SAR Auxins and Cytokinin in crown tissue Our its components as well as hyperspectral reflec­
establishment While recent studies implicate results afirm that transplants grown under far­ tance bands in a diverse soybean population
NHP in pathogen defense across angiosperms red (760nm) and blue (450nm) light emitting We have also proposed hyperspectral-wide
the functional characteriZation of SARD4 diodes at a dominant blue ratio (1 5) exhibited association study (HWAS) as a new proce­
and FMO1, and their participation in SAR in significant increase in flower bud induction dure for studying the association between
common hexaploid wheat (Triticum aestivum) compared to ratios of 5 1 and 1 1 in Fragaria hyperspectral reflectance bands and yield
is lacking Here we employed a reverse­genet­ vesca and Fragaria ananassa This dominant Using HWAS we identified five hyperspectral
ics approach to identify functional orthologs blue ratio­induced growth is accompanied reflectance bands with significant importance
of AtSARD4 and AtFMO1 in wheat Gene and by significant increase in concentrations of values suitable for association analyses The
protein homolog y searches and transcriptome flowering stimulators Gibberellins (GA1 and results demonstrate the outperformance of
analyses yielded 48 TaFMO1 and 3 TaSARD4 GA7) and Cytokinin (kinetin) in both species SVR­mediated GWAS over conventional GWAS
candidates Representatives were selected Auxin concentrations was higher for F. vesca in methods through the identification of admis ­
and two TaFMO1 candidates were successfully response to dominant blue light but an equal sible yield­related QTL using yield component
expressed in a SAR­deficient Arabidopsis fmo1 ratio (1 1) resulted in the highest concentration traits and selected hyperspectral reflectance
deletion mutant These transgenic TaFMO1 in F. ananassa From these results it can be bands In this presentation the results of this
Arabidopsis lines revealed a potential partial suggested that light quality controls flower study will be discussed in more detail and
recover y in SAR to oomycete Hyaloperonospo­ bud induction by its efect on gibberellins we hope the information will be beneficial to
ra arabidopsidis indicating possible functional and cytokinin Expression of flowering related soybean breeders for developing high­yield
complementation Similarly TaSARD4­express­ genes were significantly higher in dominant cultivars with improved genetic gains
ing transgenic Lsard4 Arabidopsis lines are blue light 1 week afer treatment (WAT)
being generated Preliminar y trials of wheat followed by dominant far­red (6­WAT) Also a 8. Unravelling RNA helicases in Ustilago
supplemented with 1mM Pip and 10mM NHP dominant blue ratio activates the expression maydis and their role during teliospore
significantly enhanced wheat resistance to of both FvFT1 and FvTFL1 gene at an earlier dormancy and germination
two fungal pathogens Blumeria graminis f sp stage although flowering seemed to occur A M SETO AND B J SAVILLE Environmental
tritici (powder y mildew) and Puccinia triticina independently of FvTFL1 and Life Sciences Graduate Program, Trent
(wheat leaf rust) Evaluating levels of Pip and University, 1600 West Bank Drive, Peterborough,
NHP in the transgenic Arabidopsis lines and 7. Using Hyperspectral­Wide Associa­ ON, K9L 0G2, Canada; (B.J.S.) Forensic Science
in wheat when challenged by pathogens is tion Study (HWAS) and machine learn­ Program, Trent University, 2140 East Bank Drive,
in­progress using HPLC­MS We are exploiting ing­mediated Genome­Wide Association DNA Building, Peterborough, ON, K9L 0G2,
the vast knowledge emanating from Arabidop­ Study (GWAS) for identifying yield QTL in Canada.
sis research to draw parallels with wheat as soybean from reflectance bands and yield
to improve disease resistance in a ma or crop component traits The emergence of fungal diseases is a ma or
plant

/ 29