Page 32 - TriSociety Virtual Event

Abstracts

Session 4. Student oral compe­ sis as an environmentally friendly approach 17. Cannabis inflorescence yield and can­
tition: Innovations for Pre­ and in the implementation of an integrated pest nabinoid concentration are not improved
Post­harvest Quality management program with long­term exposure to UV radiation
V RODRIGUEZ­MORRISON D LLEWELLYN AND
16. Pre­harvest spray hexanal enhances Y ZHENG School of Environmental Sciences,
15. Preharvest UV treatment a potential the post­harvest quality of ‘Honeycrisp’ University of Guelph, Guelph, ON N1G 2W1,
ecological approach for the control of apples by regulating membrane degrada­ Canada.
Xanthomonas campestris pv. vitians indu- tion­associated genes
ces key genes associated with homeosta­ K SRISKANTHARAJAH W EI KAYAL A J It is commonly believed that exposing Can­
sis, growth, and defense in lettuce SULLIVAN G PALIYATH AND J SUBRAMANIAN nabis sativa L. (cannabis) plants to ultraviolet
A SIDIB( M T CHARLES O NICOLAS AND Department of Plant Agriculture, University of (UV) radiation can enhance L ­tetrahydrocan­
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C BEAULIEU (A.S., M.T.C., C.B) Department of Guelph, Guelph ON N1G2W1, Canada; (W.K) nabinol (L ­THC) concentrations in female
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Biology, Université de Sherbrooke, 2500 Bou­ Faculty of Agricultural and Food Science, inflorescences However a lack of published
levard de l’Université, Sherbrooke, QC J1K 2R1, American University of Beirut. P.O. Box 11-0236, scientific studies has lef knowledge­gaps in
Canada; (A.S., M.T.C.) Saint-Jean-sur-Richelieu Riad El Solh, Beirut, Lebanon; (J.S) Department the efects of UV on cannabis In this study we
Research and Development Centre, Agriculture of Plant Agriculture, University of Guelph-Vine­ investigated the efects of UV exposure level
and Agri-Food Canada, 430 Boulevard Gouin, land Station, 4890 Victoria Ave N, Vineland, ON on photosynthesis growth inflorescence
Saint-Jean-sur-Richelieu, QC J3B 3E6, Canada; L0R2E0, Canada. yield and secondar y metabolite composi­
(O.N.) Harrow Research and Development tion of two indoor­grown cannabis cultivars
Centre, Agriculture and Agri-Food Canada, 2585 'Honeycrisp' (Malus domestica Borkh ) a 'Low Tide' (LT) and 'Breaking Wave' (BW)
County Road 20, Harrow, ON N0R 1G0, Canada. premium apple cultivar is highly suscepti­ Afer growing vegetatively for 2 weeks under
ble to bitter pit during post­har vest storage a canopy­level photosynthetic photon flux
Lettuce (Lactuca sativa L ) is one of the most Hexanal has been shown to enhance the shelf density (PPFD) of :225 fmol m s in an
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widely consumed leafy vegetables in the life of many temperate and tropical fruits Here 18­h light/6­h dark photoperiod plants were
world Its susceptibility to bacterial leaf spot we report the efects of hexanal on post­har­ grown for 9 weeks in a 12­h light/12­h dark
(BLS) caused by Xanthomonas campestris pv vest shelf life including bitter pit disorder in "flowering' photoperiod under a canopy­level
vitians (Xcv) can result in 100% yield losses 'Honeycrisp ' 'Honeycrisp' trees grown in a PPFD of :400 fmol m s and 3 5 h d of
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Despite the excessive and repeated use of commercial orchard in the Niagara region supplemental UV radiation with UV photon
pesticides control of this disease remains di­ were sprayed with hexanal and compared flux densities (UV­PFD) ranging from 0 01 to
ficult Consumers concerned about human­ against har vista� and non­treated control 0 8 fmol m s provided by light­emitting
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health and environmental protection denoun­ Apples were har vested at the commercial diodes (LEDs) with a peak wavelength of 287
ce the presence of residues on horticultural harvest day and stored at 2 5 C for four nm The severity of UV­induced morpholog y
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products and the abusive use of pesticides in months Both hexanal and har vista� treated and physiolog y symptoms worsened as UV
agriculture The search for new and efective fruits showed lower ethylene production and exposure level increased Dr y inflorescence
biological approaches is need to improve the decreased physiological loss in weight com­ yield decreased with increasing UV exposure
control of BLS In the present study a series of pared to control Further hexanal treatment level in LT but not in BW In LT total equivalent
independent trials were conducted to evalua­ significantly reduced the occurrence (8­14%) L ­THC and total equivalent cannabidiol (CBD)
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te the repeated use of a hormetic dose of 0 4 severity (7­10%) and progression (8­19%) concentrations decreased with increasing
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kJ/m of UV­C radiation to control Xcv and to of bitter pit compared to the har vista� and UV exposure level whereas there were no UV
assess the impact of this treatment on lettuce control respectively Hexanal also decreased treatment efects on total equivalent concen­
yield This study showed a 30­50% reduction the phospholipase­D (PLD) enZyme activity trations of individual cannabinoids in BW The
in lettuce susceptibility to BLS as a function throughout the storage compared to the other potential for using UV to enhance cannabis
of the number of c ycles of UV­C treatment two treatments qRT­PCR was conducted to quality must still be confirmed before it can
without negative efects on agronomic charac­ study the expression profiles of four PLD and be used as a production tool for modern
teristics and with an increase in total mineral six calcium/calmodulin­related genes poten­ indoor­grown cannabis cultivars
concentrations shelf life and dr y mass Data tially involved in membrane degradation and
revealed diferential expression of genes asso­ cellular calcium modulation on post­har vest 18. Compost amendments affect plant
ciated with homeostasis growth and defense quality Hexanal significantly decreased the performance and crop quality without
We conclude that UV­C hormesis under the expression of MdPLDa1, MdCNGC14-like and affecting disease severity in a long­estab­
conditions described in the present study MdCAM4 throughout the storage compared to lished crown gall­diseased vineyard.
is an efective eustress that will not interfere other treatments These findings indicate that P MCGONIGAL T M VOEGEL C DOUGL AS
with normal growth or the ability of plants to a delay in the membrane degradation process AND L M NELSON University of British Colum­
defend themselves against other potential caused by hexanal modulates the expression bia, Okanagan Campus, 1177 Research Road,
stressors The knowledge gained from this of the above genes and thus enhances the Kelowna, BC V1V 1V7, Canada; (C.D.) Quails’
work could promote pre­har vest UV­C horme­ post­har vest quality of 'Honeycrisp ' Gate Estate Winery, 3303 Boucherie Road,
Kelowna, BC V1Z 2H3, Canada.

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