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incompatible and compatible interactions, in three genomic regions on chromosomes 1 14. A single base extension assay for
respectively. The susceptible Westar was used 9 and 14 These data along with the progress pathotyping clubroot [Plasmodiophora
as the control targeting key time points in the­ achieved in the candidate genes characteriZa­ brassicae] of canola
se interactions and analyzed by dual RNA­seq. tion will be presented and discussed H. H. TSO, L. GALINDO­GONZÁLEZ AND S. E.
L. maculans is a hemibiotrophic fungus which STRELKOV. Department of Agricultural, Food
starts its life cycle as a biotroph and turns 13. Gibberellin regulation of protein and Nutritional Science, 410 Agriculture/Forest­
into a necrotroph later. Out of phytohormone accumulation in developing pea (Pisum ry Centre, University of Alberta, Edmonton, AB
composition, salicylic acid (SA), jasmonic sativum L.) seeds T6G 2P5, Canada.
acid (JA), and ethylene (ET) play vital roles J D GIEBELHAUS J A OZGA AND D M REI­
in plant­pathogen interactions and trophic NECKE Plant BioSystems Division, Department Clubroot caused by the soilborne pathogen
switch of the pathogen. Genes related to host of Agricultural, Food, and Nutritional Science, Plasmodiophora brassicae is one of the most
SA metabolism, phytohormone considered University of Alberta, Edmonton, AB, T6G 2P5, detrimental diseases of canola (Brassica
against biotrophs, upregulated early stage of Canada. napus) in Canada Several host diferential sets
the incompatible interaction compared to the have been developed for pathotype iden­
compatible interactions. In contrast, genes of Many field pea varieties have a mutation in tification including the Canadian Clubroot
JA and ET metabolism, which are considered the PsGA3ox1 gene which causes a decrease in Diferential (CCD) set However pathotyping
to be high against necrotrophs, were signi­ bioactive gibberellins (GA s) a plant hormone based on the reaction of host diferentials is
ficantly higher in later stages of compatible that regulates growth and de velopment This time­consuming labor­intensive and requires
interactions. However, the genes of JA and mutation in field pea leads to lower GA le vels biosecure greenhouse facilities To facilitate
ET metabolism were several times lower at producing shorter stemmed plants useful for rapid pathotype identification a highly spe­
later time points in incompatible interaction cultivation; however its efects on seed com­ cific assay was developed to detect clusters
suggesting the L. maculans incompatible inte­ position are not well understood This study corresponding to P. brassicae pathotypes 3H
raction remain under control at 7­ and 11­ dpi tests the hypothesis that part of GA s efect and 5X This assay is based on SNaPshot a
and the pathogen remains more as a biotroph on seed development is through modulation single base extension (SBE) reaction Primers
without switching into necrotrophic stage. of protein accumulation in the de veloping are designed upstream of the discriminating
seeds Using GA overproducing and isogenic polymorphic base and extended by one base
12. Identification and characterization of null control lines changes in seed tissue free with fluorescently labeled dideoxynucleotides
novel powdery mildew resistance genes amino acid and total nitrogen content were (ddNTPs) when the respective template is in
in flax determined to identify potential GA­induced the mix A conventional polymerase chain re­
V CLEMIS M ZAIDI S CLOUTIER AND B FOFA­ efects on processes that afect protein accu­ action (PCR) primer pair was designed to pro­
NA Charlottetown Research and Development mulation during seed de velopment Cotyle­ duce the 304 base pair amplicon to be used as
Centre, Agriculture and Agri-Food Canada, don nitrogen content per seed and estimated template for the SBE reaction and a SNaPshot
Charlottetown, Prince Edward Island, Canada. protein content were elevated in the GA­over­ primer was designed for pathotyping The
(S.C) Ottawa Research and Development Cen­ producing line during de velopment and at SNaPshot primer was optimiZed on individual
tre, Agriculture and Agri-Food Canada, Ottawa, maturity compared to the null line suggesting templates from single­spore isolates provi­
Ontario, Canada. that seed storage protein accumulation is ding the expected base resolution and then
influenced by GA Developmental variation in assessed for its limit of detection in template
Powder y mildew (PM) caused by Podosphae­ the profiles of key free amino acids involved proportions of 10 90 20 80 30 70 40 60 and
ra lini, is a common fungal disease that can in seed nitrogen transport and storage in 50 50 Additional testing was carried out on
lead to reduced flax seed quality and yield seed coat endosperm and cotyledon seed DNA extracted from canola root galls collected
Currently howe ver the causative resistant tissues indicate that GA could potentially from the field to evaluate the sensitivity of the
genes are not well known and characteriZed regulate amino acid transport and meta­ assay against field isolates The assay was able
The ob ective of this study is to identify the flax bolism dynamics within de veloping seeds to detect a 10% relative allelic proportion in a
genomic regions associated with PM resistan­ These modifications in turn could influence 10 90 template mixture with both single ­spore
ce for a detailed identification and characteri­ the rate of storage protein synthesis in the isolates and field isolates The application
Zation of PM resistance genes A recombinant cotyledons with possible implications on final of this technique appears promising for
inbred line (RIL) flax population derived from seed protein content The knowledge gained large­scale SNP pathotyping to produce highly
a cross between Linda (moderately resistant) on GA regulation of storage protein produc­ sensitive results
and Norman (susceptible) was used for PM tion during seed de velopment can be used
phenotyping genotyping and a Genome Wide to improve protein content in conventional
Association (GWAS) mapping PM phenotypic field pea varieties which could address issues
data showed a normal distribution indicating faced by global agriculture and the plant­pro­
a quantitative inheritance of the PM resistance tein industry
trait and the GWAS identified significant SNPs

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