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91. The role of drought stress on the 92. Effects of two bacterial inoculants on Soybean [Glycine max (L ) Merr ] is the leading
acquisition of freezing tolerance in as­ the physiology and transcription of osmo­ legume crop in the world It fixes atmospher­
paragus (Asparagus officinalis L.) under protectant related genes in four genera­ ic nitrogen through the symbiotic rhiZobia
controlled conditions tions of alfalfa breeding lines bacteria that inhabit root nodules Drought

W SHORT AND D J WOLYN Department of S LUNDELL AND B BILIGETU Plant Science stress limits plant growth yield and symbiotic
Plant Agriculture, University of Guelph, Guelph, Department, University of Saskatchewan, Sas­ nitrogen fixation (SNF) in soybean The main
ON, N1G 2W1, Canada. katoon, Saskatchewan, S7N 5A8 Canada. ob ective of this study is to identify allelic vari­
ation associated with SNF in a short­season
Cold acclimation induces freeZing tolerance Soil salinity is a malady inhibiting crop growth soybean diversity panel under drought stress
in asparagus (Asparagus officinalis L ) which is on 397 million ha of land worldwide and 3 75 A diversity panel of 100 early­maturity Canadi­
important for sur vival and longevity in temper­ million ha in Western Canada Planting deep an soybean varieties for which genome­wide
ate climates Previous studies indicated that rooted perennial forages such as alfalfa in genotypic data are available was used in this
cultivar "Guelph Millennium'(GM) adapted to salt afected land can act both as a means of study A greenhouse pot experiment was con­
Southern Ontario acquired freeZing tolerance land remediation by lowering the salt­laden ducted to determine plant phenotypic traits
earlier in the fall and maintained it later in the groundwater table and as a supplemental and SNF under drought stress Seedlings were
spring than "UC157'(UC) a cultivar common­ revenue stream for producers Increased inoculated with Bradyrhizobium japonicum
ly grown in desert climates Since the cold production of osmoprotectants is one means USDA 110 and the initial soil moisture content
acclimation and drought response pathways of raising the salinity tolerance of a plant was maintained at 80% field capacity The
in plants overlap with common cellular Mutualistic soil bacteria as well as selection for drought treatment was imposed afer 3­weeks
responses the ob ective of this research was salt tolerant plant genotypes have both been of plant growth where half of the plants were
to determine if drought alone induced freeZing demonstrated to be mechanisms capable of maintained at 30% field capacity and the rest
tolerance or interacted synergistically with increasing the production of anti­stress related at 80% field capacity until maturity Results
cold temperatures Seedlings of GM and UC compounds in plant tissues This experiment showed a reduction in stomatal conductance
were sub ected to control drought or cold aims to explore whether 2 strains of soil bac­ number of pods per plant number of seeds
acclimating conditions for six weeks Addition­ teria (Ensifer meliloti, Halomonas maura) can per plant and seed yield whereas an increase
al treatments included six weeks of drought or increase concentrations of osmoprotectants in in leaf chlorophyll content under drought
cold conditions followed by six weeks of cold alfalfa whether salt­adapted genotypes from a stress Genotypic variability was found for leaf
acclimation and plants grown for 12 weeks multi­generation breeding program targeting chlorophyll content stomatal conductance
under control conditions LT the tempera­ salinity tolerance produce greater concen­ photosynthesis (at flowering ) number of
50
ture at which 50% of plants die and metab­ trations of osmoprotectants and whether pods per plant number of seeds per plant
olites/parameters associated with freeZing soil bacteria can interact with salt­adapted and seed yield (at maturity) The % nitrogen
tolerance were measured GM had decreased genotypes to produce more osmoprotectants derived from the atmosphere (%NDFA) will
LT levels (increased freeZing tolerance) compared to earlier generations in 0 8 and be measured using the N­dilution method
15
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compared to UC under both drought and 16 ds/m soils If increased levels of osmo­ A genome­wide association study will be per­
cold acclimating conditions with no cultivar protectants are found among treatments an formed to identify allelic variation associated
diference obser ved under control conditions rt­qPCR study will be conducted to deduce the with SNF under drought stress and provide
Drought stress prior to cold acclimation cause(s) of increased osmoprotectant produc­ molecular markers useful in future soybean
increased LT (reduced freeZing tolerance) in tion among treatments This study will aid in breeding programs
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UC and no synergism was obser ved for GM adapting alfalfa cultivars to growth on saline
High root shoot ratio and low crown percent soil thereby producing a revenue stream for 94. Breeding for improved nitrogen fixa­
water was associated with increased freeZing producers from marginal agricultural land tion in pea
tolerance in GM while a high crown sucrose L DHILLON D LINDSAY C YANG J D KNIGHT
concentration in UC was related to decreased 93. Understanding the genetic basis for AND T D WARKENTIN Department of Plant
freeZing tolerance for the drought treatment nitrogen fixation in early maturity soy­ Sciences, University of Saskatchewan, 51 Cam­
Overall drought stress induced cultivar bean under the drought stress pus Drive, Saskatoon, S7N 5A8, Canada; (C.Y.)
specific adaptations causing diferences in D K D KOMBALA LIYANAGE D TORKAMA­ Swift Current Research and Development Cen­
freeZing tolerance thus confirming the inter­ NEH F BELZILE L GORIM G CHEN AND M S tre, Agriculture Agri-Food, 1 Airport road, Swift
relationship between these two physiological THILAKARATHNA Department of Agricultural, Current, S9H 3X2, Canada; (J.D.K.) Department
pathways Food and Nutritional Science, University of of Soil Science, University of Saskatchewan, 51
Alberta, 116 St & 85 Avenue, Edmonton, AB T6G Campus Drive, Saskatoon, S7N 5A8, Canada.
2R3, Canada; (D.T) Département de Phytolo­
gie, Université Laval, Pavillon Paul-Comtois The process of biological nitrogen fixation
2425, rue de l’Agriculture, Québec QC G1V 0A6 (BNF) through nodules of legumes benefits
Canada; and (F.B) Département de Phytologie, soil fertility increases crop productivity and re­
Université Laval, 2325, rue de l’Université Qué­ duces the requirement for chemical fertiliZers
bec, QC G1V 0A6, Canada. Pea is an important pulse crop in western Ca­

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